Translational_Unit

Part:BBa_K917000

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-19)

cscA sucrose hydrolase from E. coli 157:H7 Sakai

The is the gene cscA (Ecs3243) from Escherichia coli O157:H7 strain Sakai, encoding a sucrose hydrolase gene (Jahreis, et al., 2002). K12-derived laboratory strains of E. coli generally can not use sucrose as a carbon source, but it has been reported that expression of this gene alone from the related strain E. coli W is sufficient to allow good growth with sucrose as sole carbon source. This BioBrick includes the native ribosome binding site (overlapping the scar sequence). This biobrick can be used as a selectable marker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None